|Season 1, Episode Special|
- Mickey Mouse- Entertainment superstar and general manager/co-owner of the House of Mouse. Mickey is most visible as the club's flamboyant theater host and, in practice, leaves most of the club's management to the other staff.
- Minnie Mouse- Mickey's girlfriend, show planner and bookkeeper. Minnie is responsible for much of the club's day-to-day administration. Her cool, rational personality has kept the club running in times of crisis.
- Donald Duck- Mickey's best friend and assistant manager/co-owner of the House of Mouse. Donald is responsible for the overall customer service at the club and attends to the needs of the club's VIP guests. He secretly covets Mickey's fame and position at the club.
- Daisy Duck- Donald's girlfriend and reservation clerk. Somewhat frivolous, Daisy harbors ambitions to perform on-stage at the club with various acts.
- Goofy- Mickey and Donald's friend, Max's father, and head waiter of the club. Clumsy yet lovable, Goofy manages the club's restaurant operations.
- Pluto- Mickey's pet dog and club mascot.
- Horace Horsecollar- Mickey's long-time friend and technician. Horace runs and maintains the club's technical equipment including lights, loudspeakers, and video players.
- Clarabelle Cow- Minnie's long-time friend and gossip columnist. Clarabelle collects gossip and rumors from all over Toontown for her talk show at the club. Her gossip has occasionally lead to problems for the club's staff.
- Max Goof- Goofy's son and valet parking attendant.
- Huey, Dewey, and Louie- Donald's three nephews and a house band with alternating names such as "The Quackstreet Boys", "Quackwork", "Kid Duck" and "The Splashing Pumpkins".
- Gus Goose- Donald's cousin and executive chef of the club. A notorious glutton, Gus sometimes eats the food he prepares for the guests just before it is served.
- Magic Mirror- Magical artifact and on-site consultant. Mickey and his friends sometimes ask for advice from the Magic Mirror on a variety of issues. He is also available for queries from the club's guests. Unsurprisingly, the Evil Queen can sometimes be found staring at the Mirror.
- Mike the Microphone- A talking microphone and announcer. He usually assists Mickey in presenting and ending events on-stage nightly.
- Penguin waiters- Waiting staff of the club. According to an advertisement at the club, they are also available for hire on a temporary basis.
- Animated brooms- Janitorial staff of the club.
- Pete- The club's greedy landlord, who often attempts to shut the theater down for his own personal gain by sabotaging the show, since Mickey's contract states that the club stays open only for as long as the show goes on.
|1||"The Stolen Cartoons "||January 13, 2001||
In an attempt to end the show and shut down the House of Mouse, Pete steals all the cartoons. Meanwhile, Donald is put in charge of the club after Mickey and Goofy leave to film a new cartoon. Pete is eventually found out when he tries to point out the new cartoon is the only one they have, accidentally letting the others fall out of his coat.
Cartoons: Pluto Gets the Paper: Wet Cement (2001), Donald's Dynamite: Magic Act (2000), Hickory Dickory Mickey (2001)
|2||"Big Bad Wolf Daddy "||January 27, 2001||
Donald hires the Big Bad Wolf and the Three Little Pigs to perform a show at the club, which Mickey fears since the wolf damaged the club the last time he performed. The Three Pigs confront the Wolf at the end for blowing everyone away. However the doors fall onto the pigs, forming a house. This causes the wolf to blow the house, including the club, down.
Cartoons: Donald's Charmed Date (2001), Pluto Gets the Paper: Mortimer (2000), How to Be Groovy, Cool, and Fly (2001)
|3||"The Three Caballeros "||February 3, 2001||
The Three Caballeros are scheduled to perform at the club, but no one remembers that Donald was a Caballero. Determined to not be forgotten, he tries to be the most important performer of the group, re-naming himself "The Duck Formerly Known as Donald" (an obvious parody of Prince).
Cartoons: Donald's Fish Fry (2000), How to Be Smart (2001)
|4||"Goofy's Valentine Date "||February 10, 2001||
Cartoons: Donald's Valentine Dollar (1999), Mickey to the Rescue: Staircase (1999), Pluto's Arrow Error (1999)
|5||"Timon_and_Pumbaa"||February 17, 2001||
Timon and Pumbaa are set to perform, but end their friendship after they argue over what they are going to do in their act. Now it's up to Mickey and Donald to bring them back together.
Cartoons: Pluto's Magic Paws (2000), Mickey to the Rescue: Cage and Cannons (1999), Golf Nut Donald (2001)
|6||"Jiminy Cricket"||February 24, 2001||
Jiminy Cricket quits his job as Pinocchio's conscience after Pinocchio befriends Pain and Panic and decides to become Mickey's conscience. Mickey quickly realizes that Pinocchio and Jiminy must stay together and has to figure out how to do it.
Cartoons: Mickey's Mistake (1999), Daisy's Road Trip (1999)
|7||"Unplugged Club "||March 3, 2001||
Pete attempts to stop the show again by removing the electrical battery and turning off all of the club's electricity. Luckily, Mickey and Minnie turns it back on again with Zeus' lightning bolt.
Cartoons: Music Store Donald (2001), Mickey's Cabin (2000)
|8||"Gone Goofy "||March 10, 2001||
Donald tries to get Goofy fired from his job as head waiter after the club's budget causes problems.
Trivia: The Horned King makes an appearance in this episode.
Cartoons: Pit Crew (2001), Goofy's Extreme Sports: Shark Feeding (2000), Donald's Goofy World (2001)
|9||"Rent Day "||March 17, 2001||Before the show ends, Mickey needs to pay the rent for Pete after he unwittingly spends the money on cheese.
Cartoons: Mickey's Mountain (2000), Maestro Minnie: Circus Symphony (2001), Big House Mickey (2001)
|10||"Donald's Lamp Trade "||March 24, 2001||Jafar tricks Donald into thinking that Mickey is not his friend so he can steal a lamp for him.
Cartoons: Survival of the Woodchucks (2000), Goofy's Radio (2000)
|11||"Donald's Pumbaa Prank "||March 31, 2001||
It's April Fool's Day, and after Mickey plays a friendly prank on Donald, Pete takes advantage of Donald and convinces him to play a nasty prank that could shut down the club for good.
Cartoons: Mickey's April Fools (2001), Whitewater Donald (2000)
|12||"Thanks to Minnie "||November 8, 2004||Minnie feels that she is not appreciated at the House of Mouse after Clarabelle starts a rumor, so she quits her job, making the rest of the staff realize how much she means to them.
Cartoons: Minnie Visits Daisy (1999), Mickey's Big Break (2000)
|13||"Pluto Saves the Day "||November 29, 2004||Pluto has to save the club after Pete gives magical sleeping apples to the staff in another attempt to shut down the club.
Cartoons: Goofy's Big Kitty (1999), Pluto's Kittens (2000)
|14||"Daisy's Debut"||September 22, 2001||
Daisy is given a chance to perform a duet with Mickey at the club, which makes Minnie feel left out.
Cartoons: Daisy's Big Sale (2001), Topsy Turvy Town (1999)
|15||"Goofy for a Day"||September 29, 2001||
Max thinks that Goofy's job isn't as important as Mickey and Donald's, so Goofy challenges Max to be the head waiter.
Cartoons: How to Be a Waiter (1999), Maestro Minnie: Hungarian Rhapsody No. 6 (1999), Donald's Dinner Date (1999)
|16||"Clarabelle's Big Secret"||October 6, 2001||
When everyone is tired of Clarabelle's old gossip, she announces that at the end of the show, she will reveal a big secret about somebody. Now, everyone in the club is convinced that she knows something hidden about them which they accidentally reveal to her.
Cartoons: How to Be a Spy (1999), Double Date Donald (2001)
|17||"The Mouse Who Came to Dinner"||October 31, 2001||
Mickey and the gang struggle to please Mortimer Mouse, who they believe is an important restaurant inspector.
Cartoons: Mickey's Mix-Up (2000), Maestro Minnie: Flight of the Bumble Bee (1999), Donald's Grizzly Guest (1999)
|18||"Max's New Car"||November 3, 2001||Max has to prove to his father that he is responsible enough to own a car.
Cartoons: Mickey's New Car (1999), Car Washers (2000), Motor Mania (1950) (clip)
|19||"Not So Goofy"||November 10, 2001||When Mickey and the gang get tired of Goofy's clumsiness, Goofy tries to become "un-Goofy."
Cartoons: Roller Coaster Painters (1999), Goofy's Extreme Sports: Wakeboarding (1999), How to Wash Dishes (2000)
|20||"Everybody Loves Mickey"||November 17, 2001||Donald gets tired of all the attention Mickey is getting and makes an alliance with Mortimer Mouse.
Cartoons: Mickey's Rival Returns (2000), Mickey to the Rescue: Train Tracks (1999), Donald's Failed Fourth (1999)
|21||"Max's Embarrassing Date"||January 19, 2002||Max brings Roxanne to the House of Mouse on a date, but is worried that Goofy will embarrass him. When he asks Mickey and the rest of the gang for help, they end up causing more trouble than Goofy.
Cartoons: Pluto's Penthouse Sweet (1999), How to Ride a Bicycle (1999)
|22||"Where's Minnie?"||January 26, 2002||Minnie goes down into the club's creepy basement to get a birthday surprise for Mickey. When she doesn't return for a while, Mickey gets worried and organizes a search party.
Cartoons: Mickey and the Color Caper (2002), Donald's Pool (2000)
|23||"Super Goof"||February 2, 2002||After eating some contaminated peanuts, Goofy transforms into "Super Goof" and must save the club from a meteor strike.
Cartoons: How to Take Care of Your Yard (2000), Locksmiths (2000)
|24||"King Larry Swings In"||February 16, 2002||King Larry, King Louie's twin brother, wreaks havoc at the House of Mouse.
Cartoons: Mickey and the Seal (1948), Goofy's Extreme Sports: Paracycling (1999), How to Be a Gentleman (2000)
|25||"Ladies' Night"||February 23, 2002||Mickey, Donald, and Goofy go bowling for the night and leave Minnie, Daisy, and Clarabelle in charge of the club.
Cartoons: Purple Pluto (1999), Daisy Bothers Minnie (1999), Maestro Minnie: William Tell Overture (1999)
|26||"Dennis the Duck"||May 18, 2002||Donald never thought that the black and white cartoons were very funny, but a vintage character called Dennis the Duck tries to win him over.
Cartoons: The Whoopee Party (1932), Pioneer Days (1930) (clip), Mickey and the Goat Man (2002)